Bumblebees, the pollinators of the 21st century

Strategies to control the risks associated with the use of commercially produced insects

The globalization of the trade of many animal species as food, of a domestic type, beneficial for pest control or pollination, facilitates the establishment of these in places outside their natural origin, in addition to the transmission of diseases between populations, both non-native and indigenous. These biological invasions contribute significantly to the alteration of ecosystem functions and the homogenization of the biota, endangering its balance and the survival of some native species.

The international trade in bumblebees exemplifies a paradoxical case, since the beneficial effects of pollination and food production conflict with the risks associated with introducing non-native species into an ecosystem, such as the transmission of diseases that affect the local wildlife.

Bumblebees were first introduced to improve poly nization of agricultural crops in New Zealand around 1900. Since the 1980s, these bumblebee hives have been mass-produced for greenhouse pollination. Thus, each year more than two million colonies are produced, with Bombus terrestris being the most commercialized species. These hives are shipped all over the world, frequently causing accidental escapes or the transmission of diseases to other insects.

Bombus terrestris is a species of Palearctic origin, but due to accidental leaks from greenhouses and intentional releases for pollination in agricultural fields, today it is present in places such as Japan, Chile, Argentina, New Zealand and Tasmania. Given the importance of the trade in pollinator hives for the global economy and for food production, it is impossible for states to prohibit their trade. Therefore, the establishment of efficient species management strategies is a priority, which should be effective in establishing areas at risk of settlement of invasive species, in this way, it could be regulated what type of pollinators are more convenient to import in each area of the world. planet, for which it is necessary to improve knowledge of pollinator species and their biology.

On the other hand, given the evidence of contagion of pathogens between commercially bred pollinators and populations of native pollinators, it is essential to define protocols for the control and monitoring of emerging diseases in pollinators, which allow the movement of hives safely. For this, it is essential to have techniques for the identification and quantification of pathogens that allow us to act effectively and quickly in the face of an infection.

Molecular biology techniques offer several advantages over traditional techniques, being very useful when detecting pathogens that are affecting a hive. The main advantages of molecular techniques are:

  • Speed. The time to perform an analysis varies from 3-4 hours to 2-3 days (depending on the analysis), in any case, the time required is much less than that used in other diagnostic techniques such as microbiology (4-15 days) .
  • Sensitivity. Genetic techniques are highly sensitive, they can detect very low loads, detecting the agent even before symptoms occur, which produces a benefit with respect to immunological techniques.
  • Specificity. Genetic tests for the identification of microorganisms are based on the detection of unique fragments in the genetic material of the target organism, that is, they detect only the genetic material of the organism sought, so they are highly specific and reliable and reduce the possibility of false positives.

An early identification of the organism causing the disease is crucial when applying a treatment and avoiding the spread of the agent to the hive or the natural environment, for which genetic techniques are a very good tool to speed up the diagnosis process and obtain a reliable result.

At IBEROGEN we work on the diagnosis of diseases that affect bees (Apis sp.) and bumblebees (Bombus sp.), offering a complete genetic and microscopic detection service for pathogens in workers, queens, hives and pollen, allowing our clients to select among our catalog, the specific analyses to your needs.

Catalog of diseases and parasites:

  • Visual Detection (Macroscopic/Microscopic):
    • Detection of Acarapis woodi.
    • Detection of Aethinia tumida.
    • Detection of Ephestia sp.
    • Detection of  Locustacarus bruchneri.
    • Detection of Mellitobia sp.
    • Detection of Nosema sp. (N. bombi, N. ceranae y N.apis), presence or absence and/or relative quantification.
    • Detection of Tropilaelaps clareae.
    • Detection of Varroa sp.
    • Detection of Vitula edmandsii.

 

  • PRC:
    • Detection of Acarapis woodi.
    • Detection of Apicystis bombi.
    • Detection of Ascosphaera apis.
    • Detection of Crithidia bombi.
    • Detection of American Foulbrood (Paenibacillus larvae).
    • Detection of European Foulbrood (Melissococcus pluton).
    • Detection of Nosema sp. (N. bombi, N. ceranae y N.apis).
    • DW virus detection.
    • ABP virus detection.
    • KB virus detection.
    • IAP virus detection.

 

  • Crops and germination:
    • Detection of aerobic mesophiles.
    • Mold and yeast detection.
    • Spore germination of Nosema sp. (feasibility test).

 

  • *If the analysis you need is not available, contact us.

 

Bibliography.

  • Brutscher LM, McMenamin AJ, Flenniken ML (2016) The Buzz about Honey Bee Viruses. PLoS Pathog 12(8): e1005757. doi:10.1371/journal. ppat.1005757
  • Lecocq, Harpke, Rasmont, and Schweiger. 2016. Improving international trade regulation by considering intraspecific variation for invasion risk an assessment of commercially trade species: The Bombus terrestris case. Conservation letters. doi : 10.1111/conl.12215.
  • European Food Safety Authority. Bee health European Food Safety Authority (europa.eu).
  • Surveillance program on the loss of bee colonies. Ministry of Agriculture, Fisheries and Food. program vigilanciaabejas2021-22_tcm30-514692.pdf (mapa.gob.es).
Manuel Rodríguez Morán
CEO & Commercial and Marketing Manager

From a life experience closely related to science and art, I try to participate in projects that excite me, giving my best as long as the energy exchange is reciprocal. My goal is to continue discovering, participating and getting excited about new goals.

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IBEROGEN ESTUDIOS AMBIENTALES SL has been a beneficiary of the European Regional Development Fund whose objective is to improve the competitiveness of SMEs and thanks to which it has launched an International Digital Marketing Plan with the aim of improving its online positioning in foreign markets during the year 2021. For this, it has had the support of the XPANDE DIGITAL Program of the Chamber of Commerce of the Official Chamber of Commerce, Industry, Services and Navigation of Alicante.